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Dr. Lingjun Li
Lingjun received her BE degree in Environmental Analytical Chemistry from Beijing Polytechnic University and a PhD degree in Analytical Chemistry/Biomolecular Chemistry from University of Illinois at Urbana-Champaign. She did three-way postdoctoral research at the Pacific Northwest National Laboratory, Brandeis University, and University of Illinois before joining the School of Pharmacy faculty in 2002. She currently holds joint appointments in the School of Pharmacy and Department of Chemistry at UW-Madison, as well as being named Charles Melbourne Johnson Distinguished Chair and Vilas Distinguished Achievement Professor.
Kellen Awarded
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John and Beverly Shrag Analytical Chemistry Outstanding Peer Award
This award honors John Schrag, former UW-Madison chemistry professor, and his wife, Beverly. It celebrates a peer-nominated graduate student, committed to pursuing excellence in teaching/mentorship, leadership, scientific excellence, and community-building throughout their tenure.
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Milestone Moment as Kellen becomes Ph.D. #50!!
Urinary Amine Metabolomics Characterization with Custom 12-plex Isobaric DiLeu Labeling
Journal for the American Society for Mass Spectrometry
Lower urinary tract symptoms (LUTS) are common in aging males. Disease etiology is largely unknown, but likely includes inflammation and age-related changes in steroid hormones. Diagnosis is currently based on subjective symptom scores, and mainstay treatments can be ineffective and bothersome. Biomarker discovery efforts could facilitate objective diagnostic criteria for personalized medicine and new potential druggable pathways. To identify urine metabolite markers specific to hormone-induced bladder outlet obstruction, we applied our custom synthesized multiplex isobaric tags to monitor the development of...
Extraction Optimization for Combined Metabolomics, Peptidomics, and Proteomics Analysis of Gut Microbiota Samples
Journal of Mass Spectrometry
Multiomic studies are increasingly performed to gain a deeper understanding of molecular processes occurring in a biological system, such as the complex microbial communities (i.e., microbiota) that reside the distal gut. While a combination of metabolomics and proteomics is more commonly used, multiomics studies including peptidomcis characterization are less frequently undertaken. Here, we investigated three different extraction methods, chosen for their previous use in extracting metabolites, peptides, and proteins, and compared their ability to perform metabolomic, peptidomic, and proteomic analysis of mouse cecum content. The methanol/chloroform/water...
Urinary metabolomic and proteomic analyses in a mouse model of prostatic inflammation
Urine
Lower urinary tract symptoms (LUTS) are common among aging men. Since prostatic inflammation is one of its etiologies, it is plausible that urinary metabolite and protein biomarkers could be identified and used to diagnose inflammation-induced LUTS. We characterized the urine metabolome and proteome in a mouse model of bacterial-induced prostatic inflammation. Mass spectrometry (MS)-based multi-omics analysis was employed to discover urinary protein and metabolite-based biomarkers. The investigation of isobaric dimethylated leucine (DiLeu) labeling on metabolites allowed metabolomics and proteomics analysis on the same liquid...