Dr. Lingjun Li

Lingjun received her BE degree in Environmental Analytical Chemistry from Beijing Polytechnic University and a PhD degree in Analytical Chemistry/Biomolecular Chemistry from University of Illinois at Urbana-Champaign. She did three-way postdoctoral research at the Pacific Northwest National Laboratory, Brandeis University, and University of Illinois before joining the School of Pharmacy faculty in 2002. She currently holds joint appointments in the School of Pharmacy and Department of Chemistry at UW-Madison, as well as being named Charles Melbourne Johnson Distinguished Chair and Vilas Distinguished Achievement Professor.

Andrew, Hannah, and Zicong successfully defends his second-year thesis background examination!

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Our third-year students are all officially dissertators! Congratulations Dylan, Jericha, Bin, Gutin, Yuan, Graham, Ashley, and Danqing!

Erythrocyte sphingolipid species as biomarkers of Alzheimer's disease

Journal of Pharmaceutical Analysis

Diagnosing Alzheimer's disease (AD) in the early stages is challenging. Informative biomarkers could be of great value for population-based screening. Metabolomics studies have been used to find potential biomarkers, but commonly used tissue sources can be difficult to obtain. The objective of this study was to determine the potential utility of erythrocyte metabolite profiles in screening for AD. Unlike some commonly-used sources such as cerebrospinal fluid (CSF) and brain tissue, erythrocytes are plentiful and easily accessed. Moreover, erythrocytes are metabolically active, a feature that distinguishes this sample source from other...

Mass spectrometry profiling and quantitation of changes
in circulating hormones secreted over time in Cancer borealis
hemolymph due to feeding behavior

Analytical and Bioanalytical Chemistry

The crustacean stomatogastric ganglion (STG) is a valuable model for understanding circuit dynamics in neuroscience as it
contains a small number of neurons, all easily distinguishable and most of which contribute to two complementary feedingrelated
neural circuits. These circuits are modulated by numerous neuropeptides, with many gaining access to the STG as
hemolymph-transported hormones. Previous work characterized neuropeptides in the hemolymph of the crab Cancer borealis
but was limited by low peptide abundance in the presence of a...

DiLeuPMP: A Multiplexed Isobaric Labeling Method for Quantitative Analysis of O-Glycans

Analytical Chemistry

As one of the most important post-translational modifications, glycosylation plays a pivotal role in many essential physiological functions, including cell recognition, signaling, and immune response. Thus, various qualitative and quantitative analytical strategies for glycomic profiling have been developed in recent decades. However, while extensive efforts have been devoted to the analysis of N-glycans, high-throughput quantitative analysis of O-glycans is often overlooked and underexplored. This is partially due to the lack of a universal enzyme for the release of O-glycans from the protein backbone. Furthermore...

Dual-Functional Ti(IV)-IMAC Material Enables Simultaneous Enrichment and Separation of Diverse Glycopeptides and Phosphopeptides

Analytical Chemistry

Recent developments and applications of quantitative proteomics strategies for high-throughput biomolecular analyses in cancer research

RSC Chemical Biology

Acetyl‑CoA flux from the cytosol to the ER regulates engagement and quality of the secretory pathway

Analytical Chemistry