Quantitative MS Strategies

We have validated several strategies for quantitative mass spectrometry studies. Among these are DiPyro, DiLeu, and SUGAR. These strategies are regularly employed for proteome profiling, neuropeptide analysis, and post-translational modification quantitation.

High-Resolution Enabled 12-Plex DiLeu Isobaric Tags for Quantitative Proteomics

Analytical Chemistry

Isobaric Multiplex Labeling Reagents for Carbonyl-Containing Compound (SUGAR) Tags: A Probe for Quantitative Glycomic Analysis

Analytical Chemistry

Quantitative Glycomic Analysis by Mass-Defect-Based Dimethyl Pyrimidinyl Ornithine (DiPyrO) Tags and High-Resolution Mass Spectrometry

Analytical Chemistry

Comparative 

Neuropeptide Study

Neuropeptide changes induced by food intake, environmental stress, etc. in crustacean models are profiled and compared in this research area. Along with tissue biopsy strategies, microdyalisis is employed to monitor neuropeptide secretion in live animals. 

Imaging Mass Spectrometry of Neuropeptides in Decapod Crustacean Neuronal Tissues

Journal of Proteome Research

Mapping of Neuropeptides in the Crustacean Stomatogastric Nervous System by Imaging Mass Spectrometry

Journal of the American Society for Mass Spectrometry

Neuropeptidomic Profiling and Localization in the Crustacean Cardiac Ganglion Using Mass Spectrometry Imaging with Multiple Platforms

Journal of the American  Society of Mass Spectrometry

Biomarker Discovery

Neuropeptide changes induced by food intake, environmental stress, etc. in crustacean models are profiled and compared in this research area. Along with tissue biopsy strategies, microdyalisis is employed to monitor neuropeptide secretion in live animals. 

Proteome-wide and matrisome-specific alterations during human pancreas development and maturation

Nature Communications

Isobaric Labeling Strategy Utilizing 4-Plex N,N-Dimethyl Leucine (DiLeu) Tags Reveals Proteomic Changes Induced by Chemotherapy in Cerebrospinal Fluid of Children with B-Cell Acute Lymphoblastic Leukemia

Analytical Chemistry

Acetyl‑CoA flux from the cytosol to the ER regulates engagement and quality of the secretory pathway

Scientific Reports

Mass Spectrometry Imaging

Mass spectrometric imaging enables scientists to obtain 2D and 3D maps of biological analytes in their native tissues without the need for antibodies. The Li Research group implements this technology to examine distribution patterns of neuropeptides, proteins, lipids, and metabolites in a variety of tissues.

Nanosecond photochemically promoted click chemistry for enhanced neuropeptide visualization and rapid protein labeling

Nature Communications

Complementary neuropeptide detection in crustacean brain by mass spectrometry imaging using formalin and alternative aqueous tissue washes

Analytical and Bioanalytical Chemistry

A Simple and Effective Sample Preparation Strategy for MALDI-MS Imaging of Neuropeptide Changes in the Crustacean Brain Due to Hypoxia and Hypercapnia Stress

Analytical Chemistry

Ion Mobility-Mass Spectrometry

Ion Mobility - Mass Spectrometry separates gas-phase ions by mass, charge, size, and shape through collisions with inert gas molecules. The Li Research Group is currently utilizing IM-MS to develop novel strategies for glycan separation, peptide identification, and structural analyses.

Sialylation fine-tunes glycoprotein structural microheterogeneity associated with Alzheimer’s disease as captured by native ion mobility-mass spectrometry

Prepress

Molecular basis for chirality-regulated Aβ self-assembly and receptor recognition revealed by ion mobility-mass spectrometry

Nature Communications

Reliable Tracking In-Solution Protein Unfolding via Ultrafast Thermal Unfolding/Ion Mobility-Mass Spectrometry

Analytical Chemistry

Capillary Electrophoresis MS

Capillary zone electrophoresis and capillary isoelectric focusing are being developed to couple with MS detection and MS imaging, both off-line and on-line, for enhanced neuropeptidomic and glycomic analysis.

Capillary electrophoresis coupled to MALDI mass spectrometry imaging with large volume sample stacking injection for improved coverage of C. borealis neuropeptidome

The Analyst

Capillary Electrophoresis-Electrospray Ionization-Mass Spectrometry for Quantitative Analysis of Glycans Labeled with Multiplex Carbonyl-Reactive Tandem Mass Tags

Analytical Chemistry

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Li Research Group

 University of Wisconsin Madison

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